Gene expression analysis of M. tuberculosis in patients with and without HIV co-infection

Group 3: Anne Skov-Johannessen s184330, Dea F. Skipper, Helene B. L. Petersen s194699, Johanne B. Overgaard s194691 and Rebecca C. Grenov

Introduction

Co-infection with tuberculosis (TB) is the leading cause of death in HIV-infected individuals.

Introduction

Data origin

Purpose of data collection

Data handling Remove irrelevant variables Change ugly variables Handle replications

Methods

Discriptive analysis

Methods

Normalization Log2

Methods

Analysis PCA Linear regression

Results - PCA

  • First PC explains 15% of the variance

  • 31 PCs needed to explain 90% of variance

Results - PCA

  • Slight partition of disease state on PC1
  • No clear partition of gender

Results - Linear Regression

Forest plot

Results - Linear Regression

Volcano plot